雏鸡免疫器官中chMDA5的表达与定位

doi:10.11843/j.issn.0366-6964.2016.07.023

Abstract

Abstract:

The objective of the present study was to examine the expression and location of chick melanoma differentiation associated gene 5 (chMDA5) in chick immune organs.Fifty health Highland white chicks were euthanized to collect bursa of Fabricius，thymus and spleen at 1，7，14，21，28，31，35，42，45，49-day-old，separately.Then，real-time fluorescence quantitative PCR，Western blot and IFA techniques were used to detect the expression of chMDA5 in chick immune organs.The results showed that the chMDA5 mRNA were examined in bursa of Fabricius，thymus and spleen at each age，chMDA5+ cells were detected in the cortex of bursal lymphoid follicles，the cortex and medulla of thymus lobule，the white pulp and red pulp of spleen.The expression pattern of chMDA5 protein in bursa of Fabricius and thymus tissue was consistent，which showed an upward trend in 1 to 7-day-old chicks and reached peak at 7-day-old，and then went down in 14 to 35-day-old with a fluctuation trend，then gradually increase and stabilized.In spleen，the protein expression of chMDA5 was increased with the increasing age in a period of about 14 days.The results indicated that the chMDA5 were expressed in chick immune organs，and there were differences in expression of chMDA5 with different ages.This study provide a scientific test basis for further exploration about the correlation between chMDA5 and chick immune function.

CLC Number:

S852.4

LUAN Ya-nan，GE Ming，LI Guang-xing，XIE Wan-qiu，YANG Gui-jun，ZHANG Rui-li. The Expression and Location of chMDA5 in Chick Immune Organs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1480-1487.

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The Expression and Location of chMDA5 in Chick Immune Organs

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